The CSS-Palm 4.0 could predict out potential palmitoylation sites for ~1,000 proteins (with an average length of ~1000aa) within two minutes. Also, with PHP and JavaScript, the online version was developed. Taken together, it was proposed that the CSS-Palm 4.0 will be a great help for experimentalists. Notably, in the fourth-generation GPS algorithm, the Particle Swarm Optimize (PSO) was integrated to improve the convergence speed and training accuracy.
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